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目的 观察输血传播病毒 (TTV)和肝炎病毒在慢性肾衰 (CRF)血液透析 (HD)患者中的流行情况和感染相关因素 .方法 应用巢式 -聚合酶链反应 (Nest- PCR)方法和双抗体夹心 EL ISA法 ,检测 90例 CRF血透患者和 2 0 8例西安地区职业献血员及 12 8例对照组血清 TTV- DNA,HBV五项 ,并分析 TTV与输血次数、HD时间、肝功能损害及其与各型肝炎病毒感染的关系 .结果 190例 CRF血透患者和 2 0 8例职业献血员中 TTV- DNA阳性率 ,分别是 46 .6 %和 16 .8% ,明显高于对照组 (P<0 .0 1) ;2 TTV- DNA阳性率与输血次数、HD时间呈显著的正相关 (r=0 .5 86 ,P<0 .0 1,r=0 .486 ,P<0 .0 1) ,而与年龄、性别、手术史均无显著相关 ;390例 HD患者中 ,检出肝炎病毒标志物阳性者 6 6例 (感染率 73.3% ) ,明显高于对照组 (P<0 . 0 1) ,其中以 HCV阳性 (感染率6 8.1% )最突出 ,HBV阳性 (感染率 18.1% )次之 ,HBV,HCV双重阳性者亦较明显 (感染率 13.6 % ) ;2 0 8例职业献血员中 ,检出肝炎病毒标志物阳性者 14例 (感染率 6 .7% ) ,与对照组(感染率 5 .4% )比较无明显差异 (P>0 .0 5 ) ;480例 TTV阳性患者中 5例合并 HBV感染 ,与 TTV阴性组相当 ,但合并HCV感染及 HCV、HBV双重感染差别显著 (P<0 .0 5 )(16 .0 % vs11.0 %及 6 .0 % vs
Objective To investigate the prevalence and infection related factors of transfusion transmitted virus (TTV) and hepatitis virus in patients with chronic renal failure (CRF) hemodialysis (HD) .Methods Nested polymerase chain reaction (PCR) Antibody sandwich ELISA method was used to detect serum TTV-DNA and HBV in 90 cases of CRF hemodialysis patients and 208 cases of professional blood donors in Xi’an area and 12 8 cases of control group. The number of TTV-transfusion, HD time, liver function Damage and their relationship with various types of hepatitis virus infection.Results The positive rates of TTV-DNA in 190 CRF hemodialysis patients and 208 blood donors were 46.6% and 16.8% respectively, which were significantly higher than those of control (P <0.01). The positive rate of TTV-DNA was positively correlated with the number of transfusions and HD time (r = 0.586, P <0.01, r = 0.486, P < There was no significant correlation between age, sex and operation history. Of the 390 patients with HD, 66 cases were positive for hepatitis B virus infection (infection rate was 73.3%), which was significantly higher than that of the control group (P <0. 0 1). Among them, HCV was the most positive (infection rate was 8.11%), HBV was the most positive (infection rate was 18.1%), HBV and HCV were also more common (infection rate 13.6%). Of the 208 blood donors, 14 were positive for hepatitis B virus (6.7%) and no significant difference compared with the control group (5.4%) (P> 0.05). Among the 480 TTV-positive patients, 5 cases had HBV infection, which was similar to that of TTV-negative group, but there was a significant difference (P <0.05) between HCV infection and HCV and HBV infection (16.0% vs11 .0% and 6 .0% vs