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目的观察血管内皮细胞在不同浓度的氧化型低密度脂蛋白(ox-LDL)诱导及诱导不同时间后内皮细胞促聚集和促黏附活性的动态改变及可能机制。方法将人脐静脉内皮细胞(HUVEC)株接种在6孔培养板,分别加入不同浓度的ox-LDL(50、100和200 mg/L)并孵育不同时间(12、24和48 h)。放射免疫法测定细胞上清液中前列环素(PGI2)和血栓素A2(TXA2)含量;Western blot检测HUVEC中组织因子(TF)、细胞间黏附分子1(ICAM-1)和血管细胞黏附分子1(VCAM-1)的表达。结果量效关系显示,ox-LDL预处理HUVEC 24 h后,ox-LDL呈浓度依赖性促进HUVEC聚集,表现为PGI2/TXA2比值显著下降(P<0.05或P<0.01);时效关系显示类似的结果,但以24 h作用最为显著;同时,TF的表达随浓度的升高呈升高趋势。此外,ox-LDL还促进HUVEC黏附,表现为ICAM-1和VCAM-1呈浓度依赖性升高。结论 ox-LDL可增强HUVEC促聚集和促黏附活性,其机制可能与其分泌的PGI2/TXA2下降及上调TF、ICAM-1和VCAM-1的表达有关。
OBJECTIVE: To observe the dynamic changes of vascular endothelial cells (ECs) promoting endothelial cell adhesion and adhesion after different concentrations of ox-LDL were induced and induced at different time points and its possible mechanism. Methods Human umbilical vein endothelial cells (HUVEC) were inoculated into 6-well plates and incubated with different concentrations of ox-LDL (50, 100 and 200 mg / L) for different times (12, 24 and 48 h). The levels of PGI2 and TXA2 in the cell supernatant were determined by radioimmunoassay. The expressions of tissue factor (TF), intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) expression. Results The dose-response relationship showed that pretreatment with ox-LDL for 24 h resulted in a significant decrease of PGI2 / TXA2 ratio (P <0.05 or P <0.01) in ox-LDL-treated HUVECs in a dose- However, the effect was most obvious at 24 h. At the same time, the expression of TF increased with increasing concentration. In addition, ox-LDL also promotes HUVEC adhesion, showing a concentration-dependent increase in ICAM-1 and VCAM-1. Conclusion Ox-LDL can enhance the aggregation-promoting and adhesion-promoting activity of HUVEC. The mechanism may be related to the decrease of PGI2 / TXA2 secretion and the up-regulation of the expression of TF, ICAM-1 and VCAM-1.