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目的分析鼠李糖乳杆菌(Lactobacillus rhamnosus GG,LGG)对黄曲霉毒素B_1(aflatoxin B_1,AFB_1)的降解能力,阐述LGG对由AFB_1引起的肝损伤的保护作用。方法利用LGG培养液、LGG上清液组、LGG菌体、热处理后上清液和热处理后LGG菌体处理AFB_1,利用高效液相色谱法测定AFB_1的残留量,分析LGG对AFB的降解能力。利用低、中和高剂量的LGG菌液灌胃由AFB_1引起肝损伤的大鼠,并以空白和阳性作为对照组,测定大鼠的血清肝功能及肝组织抗氧化指标,分析LGG对肝损伤的保护作用。结果 AFB_1降解实验表明LGG菌液对AFB_1具有显著的降解作用(P<0.05),36 h能够降解(92.01±2.02)%的AFB_1。降解作用是由菌体和LGG的代谢产物共同作用的结果。大鼠血清肝功能及肝组织抗氧化指标结果表明低、中和高剂量的LGG灌胃给药均能显著改善因AFB_1引起的大鼠肝功能和肝组织抗氧化指标异常(P<0.05)。结论 LGG对AFB_1具有良好的降解作用,且能够有效抑制因AFB_1引起的肝损伤。
Objective To analyze the ability of Lactobacillus rhamnosus GG (LGG) to degrade aflatoxin B_1 and AFB_1, and to elucidate the protective effect of LGG on liver injury induced by AFB_1. Methods AFB_1 was treated with LGG medium, LGG supernatant, LGG cells, supernatant after heat treatment and heat-treated LGG. The residual amount of AFB_1 was determined by HPLC, and the degradability of LGG to AFB was analyzed. Low, medium and high doses of LGG were used to inoculate the rats with liver injury induced by AFB_1, and the blank and the positive were used as the control group to determine the serum liver function and the anti-oxidation index of liver tissue in rats. The effects of LGG on liver injury The protective effect. Results AFB_1 degradation experiment showed that LGG solution could significantly degrade AFB_1 (P <0.05) and degrade AFB_1 (92.01 ± 2.02%) at 36 h. Degradation is the result of the interaction of the cell body with the metabolites of LGG. The serum liver function and the anti-oxidative indexes of liver tissue in rats showed that low, medium and high doses of LGG could significantly improve the abnormal liver function and liver anti-oxidative index of rats induced by AFB_1 (P <0.05). Conclusion LGG has a good degradation of AFB_1, and can effectively inhibit the liver injury caused by AFB_1.