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目的:继发现人源性新抗菌肽基因并进行功能验证后,进一步发现高度同源蛋白质家系并进行功能验证。方法:利用生物信息学分析手段对该蛋白家系各成员进行系统分析,人工合成具有代表性的不同肽段,采用比浊法和菌落计数法测定人工合成肽段的抗菌活性。结果及结论:发现了富含甘氨酸并且序列高度同源的抗菌肽家系,命名为格拉利素(G lyrich in),目前含有来自不同生物的29个成员,所有成员均无相关功能研究报道;根据多序列比对结果所合成的、代表家系中9个成员的4条多肽均有不同程度的抗菌作用。上述生物信息学分析和实验研究结果强烈提示了新抗菌肽家系的存在,其中跨越哺乳动物的抗菌肽家系的存在已得到充分证实。
OBJECTIVE: Following the discovery of a new human antimicrobial peptide gene and its functional verification, a highly homologous protein family was further identified and validated. Methods: The members of this protein family were systematically analyzed by means of bioinformatics analysis. Representative peptides were synthesized. The antimicrobial activity of synthetic peptides was determined by turbidimetry and colony counting. RESULTS AND CONCLUSION: Antimicrobial peptide families rich in glycine and highly homologous sequences were found, named G lyrichin, and currently contain 29 members from different organisms, all of which were reported without functional studies; The results of multiple sequence comparisons showed that the four polypeptides representing 9 members of the pedigree all had different degrees of antibacterial activity. The above bioinformatics analysis and experimental studies strongly suggest the existence of a new family of antimicrobial peptides, of which the presence of mammalian antimicrobial peptide families has been well documented.