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目的:研究高能冲击波对大鼠生精过程的影响作用。方法:将34只10周龄Wistar雄性大鼠分为3组,A组大鼠睾丸接受高能冲击波单次直接冲击,B组大鼠睾丸每周接受高能冲击波冲击1次,连续3次,C组为对照组。冲击剂量均为19kV×2000次。于冲击后48h、15d及30d分别取睾丸组织作病理学检查,测定细胞DNA含量以及细胞凋亡数量。结果:普通光镜观察两组均可见睾丸精曲细管内精子细胞及成熟精子数量减少,流式细胞仪测定细胞DNA含量,表明高能冲击波对生精过程的影响主要是引起睾丸组织的单倍体细胞和四倍体细胞数量下降。结论:高能冲击波对生精过程的作用可能是抑制初级精母细胞的形成和分化;多次高能冲击波冲击有诱导睾丸细胞凋亡数量增加的趋势
Objective: To study the effect of high energy shock waves on spermatogenesis in rats. Methods: Thirty-four Wistar male rats (10 weeks old) were divided into three groups. Rats in group A received a single direct shock with high energy shock waves. Rats in group B received high energy shock wave once a week for 3 consecutive weeks. For the control group. Impact dose are 19kV × 2000 times. At 48h, 15d and 30d after shock, testicular tissue was taken for pathological examination to determine the DNA content of cells and the number of apoptotic cells. Results: The number of spermatids and mature spermatozoa in the testicular seminiferous tubules was decreased in normal light microscope. The DNA content in the testis was determined by flow cytometry. The results showed that the impact of high-energy shock waves on the spermatogenesis was mainly caused by haploid The number of cells and tetraploid cells decreased. CONCLUSION: The effect of high-energy shock wave on spermatogenesis may be the inhibition of the formation and differentiation of primary spermatocytes. Multiple high-energy shock wave shocks induce the increase of testicular cell apoptosis