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为检测LRP15基因在肿瘤细胞以及处于不同发育阶段造血细胞的表达状况 ,探讨其在肿瘤发生、发展以及在白血病分型预后中可能具有的作用 ,利用NCBI提供的SAGE文库及NCI提供的正常组织及肿瘤细胞基因表达数据库 ,分析比较了LRP15基因在多种肿瘤组织、细胞系和正常组织中的表达谱 ;采用RT PCR方法检测了该基因在正常血细胞及原代白血病细胞中的表达状况。结果显示 ,LRP15在人类多种正常组织及肿瘤细胞中有表达 ;在幼稚细胞中表达阳性率高于成熟细胞 (P <0 .0 1) ,在急性髓细胞白血病中M1、M2 和M3 表达的阳性率高于其它亚型 (P <0 .0 1) ;难治组LRP15表达的阳性率有高于初治组的趋势。结论 ,LRP15基因与急性白血病及其它多种肿瘤的发生、发展密切相关 ,对急性白血病的临床分型具有重要的意义 ,可能对判断急性白血病的预后具有一定价值。
To detect the expression of LRP15 gene in tumor cells and hematopoietic cells at different developmental stages, and to explore its possible role in tumorigenesis, development and prognosis of leukemia. The SAGE library provided by NCBI and the normal tissues provided by NCI The expression profiles of LRP15 gene in various tumor tissues, cell lines and normal tissues were analyzed and compared. The expression of LRP15 gene in normal blood cells and primary leukemia cells was detected by RT-PCR. The results showed that LRP15 was expressed in many human normal tissues and tumor cells. The positive rate of LRP15 expression in naive cells was higher than that in mature cells (P <0.01). In acute myeloid leukemia, the expression of M1, M2 and M3 The positive rate of LRP15 expression was higher in the refractory group than in other subtypes (P <0.01). Conclusion The LRP15 gene is closely related to the occurrence and development of acute leukemia and many other tumors. It is of great significance to the clinical classification of acute leukemia and may be of value in judging the prognosis of acute leukemia.