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目的探讨甘糖酯(PGMS)对糖尿病大鼠肾脏细胞间黏附分子-1(ICAM-1)、血管细胞间黏附分子-1 (VCAM-1)表达的影响和保护作用。方法链尿佐菌素(STZ)诱发大鼠糖尿病。甘糖酯(52,26mg·kg-1· d-1)治疗8周,测定血糖、糖化血红蛋白、肾脏/体重,24h尿蛋白排泄率,利用免疫组织化学、逆转录聚合酶链 式反应(RT-PCR)和蛋白印迹(WesternBlot)技术检测肾脏组织ICAM-1,VCAM-1分布及表达。结果与糖 尿病模型组比较,PGMS有减轻肾脏肥大趋势;降低尿蛋白排泄率(P<0.05);免疫组化显示PGMS能明显减 少ICAM-1,VCAM-1在肾脏组织的分布;RT-PCR,Westernblot结果显示PGMS能明显降低ICAM-1, VCAM-1mRNA和蛋白水平表达,高剂量组效果优于低剂量组但无统计学差异。ICAM-1,VCAM-1表达与 肾脏肥大、尿蛋白存在部分正相关。结论PGMS能降低STZ诱发的糖尿病大鼠肾脏ICAM-1,VCAM-1mRNA和蛋白水平。
Objective To investigate the effect and the protective effect of PGMS on the expression of ICAM-1 and VCAM-1 in diabetic rats. Methods Streptozotocin (STZ) induced diabetic rats. (52,26 mg · kg-1 · d-1) for 8 weeks. The levels of blood glucose, glycosylated hemoglobin, renal / body weight and urinary protein excretion were measured by immunohistochemistry and reverse transcriptase polymerase chain reaction -PCR) and Western blotting were used to detect the distribution and expression of ICAM-1 and VCAM-1 in renal tissues. Results Compared with diabetic model group, PGMS could reduce renal hypertrophy and decrease urinary protein excretion rate (P <0.05). Immunohistochemistry showed that PGMS could significantly reduce the distribution of ICAM-1 and VCAM-1 in renal tissues. RT-PCR, Western blot results showed that PGMS could significantly decrease the expression of ICAM-1 and VCAM-1 mRNA and protein, while the effect of high-dose group was better than that of low-dose group. However, there was no significant difference between the two groups. ICAM-1, VCAM-1 expression and renal hypertrophy, urinary protein is partially positive correlation. Conclusions PGMS can reduce the renal ICAM-1, VCAM-1 mRNA and protein levels in STZ-induced diabetic rats.