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目的:制备茶多酚醇质体,并对其进行皮肤渗透性评价。方法:采用注入法制备醇质体,马尔文Zeta电位仪测定其粒径及电位,紫外分光光度法测定包封率;采用改良Franz扩散池装置,以ICR小鼠背部皮肤作为渗透屏障,进行体外经皮渗透试验。结果:成功制备了茶多酚醇质体,平均粒径(162.2±7.1)nm,Zeta电位-(34.3±3.9)mV,包封率(37.43±1.90)%。体外试验中,茶多酚醇质体的稳态渗透速率是97.27μg.cm-2.h-1,分别是茶多酚30%乙醇溶液、脂质体、水溶液的2.86,7.47,11.98倍,24 h累积渗透量排序为:茶多酚醇质体>30%乙醇溶液>脂质体>水溶液。结论:醇质体的制备方法可行,醇质体能够促进茶多酚的皮肤渗透。
OBJECTIVE: To prepare tea polyphenols and evaluate their skin permeability. Methods: Ethosomes were prepared by injection method. The particle size and potential were determined by Malvern Zeta Potentiometer. The encapsulation efficiency was determined by ultraviolet spectrophotometry. The modified Franz diffusion cell device was used to penetrate the skin of the back of ICR mice in vitro Transdermal penetration test. RESULTS: Tea polyphenols albumen was successfully prepared. The average particle size was (162.2 ± 7.1) nm, Zeta potential was (34.3 ± 3.9) mV, encapsulation efficiency was 37.43 ± 1.90%. In vitro, the steady-state permeation rate of tea polyphenols was 97.27μg.cm-2.h-1, which were 2.86, 7.47, 11.98 folds of the polyphenol 30% ethanol solution, The accumulative permeation amount in 24 h was: polyphenols,> 30% ethanol> liposome> aqueous solution. Conclusion: The preparation of ethosomes is feasible. Ethosomes can promote the skin penetration of tea polyphenols.