目的探讨病理技术HE染色在病理诊断中的应用。方法将2015年6月至2016年6月间我院病理科接收的100片石蜡片为本次研究对象,对所有石蜡片染色标本在显微镜下评估。结果病理技术改进后的染色标准率显著优于病理技术改进前,组间数据比较存在统计学差异,P<0.05。结论不同的组织病理通过不同的HE染色、不同的脱水和固定时间以及制片,能不痛程度的影响诊断的准确率。 Objective To investigate the application of pathological HE staining in pathological diagnosis. Methods 100 paraffin tablets received from our hospital from June 2015 to June 2016 were selected as the study objects. All paraffin-embedded specimens were evaluated microscopically. Results The improved standard staining rate was significantly better than that before the pathological improvement. There was significant difference between the two groups (P <0.05). Conclusion Different histopathological changes can affect the accuracy of diagnosis through different HE staining, different dehydration and fixation time, and film preparation.