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TiO_2 photocatalysts loaded with V_2O_5 were prepared via a modified hydrolysis process, and characterized by X-ray diffraction, transmission electron microscopy, Raman spectra and diffuse reflectance UV-Vis spectra measurements. The photocatalytic activity of V_2O_5/TiO_2 was investigated by employing splitting of water for O_2 evolution. The results indicate that V_2O_5 loading can pronouncedly improve the photocatalytic activity of TiO_2 with Fe3+ as an electron acceptor under UV or visible light irradiation. The optimum mass fraction of the loaded V_2O_5 is 8%, and the largest speed of O_2 evolution for 8%V_2O_5 (mass fraction) loaded TiO_2 catalyst is 118.2 (μmol/(L·h) under UV irradiation, and 83.7 μmol/(L·h) under visible light irradiation.