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培养皿铺胶原和培养基中加入2％二甲亚砜(DMSO)均可延长培养肝细胞的稳定存活时间,并能改善其储存糖原和维持细胞色素P450含量的能力。DMSO还能基本维持细胞呈立方球形。这个培养体系有利于观察药物对肝细胞的长期效应。
Petri dish collagen and adding 2% dimethylsulfoxide (DMSO) to culture medium can prolong the stable survival time of cultured hepatocytes and improve their ability to store glycogen and maintain the content of cytochrome P450. DMSO can basically maintain the cells were cubic spherical. This culture system is conducive to observe the long-term effects of drugs on liver cells.