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目的探索帕金森病外科治疗的移植方法，并为培养交感神经节移植脑内能否长期存活寻找组织学证据。方法用１甲基－４苯基－１，２，３，６－四氢吡啶（ＭＰＴＰ）诱发猴帕金森病模型１１只，将自体腓神经培养２４～２８天所得的雪旺细胞与神经节组织共同培养后行脑尾状核移植，观察２年后取标本，用乙醛酸诱发多巴胺荧光和免疫组织化学方法检测。结果猴帕金森病症状明显好转，移植２年后脑尾状核仍可见存活的神经节细胞，移植物与脑组织间有轴、树突联系，多巴胺荧光及神经丝蛋白、突触素、铬粒素等染色阳性。结论神经节与雪旺细胞共同培养后移植脑内可存活２年，是治疗帕金森病的较好方法。组织学观察结合荧光及免疫组织化学染色结果是判定移植物存活与否的重要手段。
Objective To explore the surgical treatment of Parkinson’s disease transplantation, and to sympathetic ganglion transplantation for long-term survival of the brain looking for histological evidence. Methods Eleven mice with Parkinson’s disease were induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Schwann cells cultured with autologous peroneal nerve for 24-28 days were cultured with ganglion Tissue culture co-cultured brain caudate nucleus transplantation, observed after 2 years to take samples, with glyoxylate-induced dopamine fluorescence and immunohistochemical detection. Results The symptoms of Parkinson’s disease in monkey were obviously improved. The surviving ganglion cells were still visible in the caudate nucleus after 2 years of transplantation. There were axial and dendritic connections between graft and brain tissue, dopamine fluorescence and neurofilament, synaptophysin, Su-positive staining. Conclusion Ganglion cells co-cultured with ganglion cells may survive for 2 years after transplantation, which is a better method for the treatment of Parkinson’s disease. Histological observation combined with fluorescence and immunohistochemical staining is an important means to determine the survival of the graft.