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目的:建立高效液相色谱法(HPLC)测定天麻含片中天麻苷(gastrodin)含量的方法,以期为天麻含片建立质量控制标准提供依据。方法:采用HPLC,以gastrodin为对照品,ZorbaxSB-C18键合硅胶柱(4.6mm×150mm,5μm),0.05%磷酸:乙睛(3∶97)为流动相,流速1.0ml.min-1,柱温为室温,检测波长220nm。结果:天麻苷在0.049~0.396 ml-1范围与峰面积呈良好线性关系,回归方程A=1.71×106+5.864×107,r=0.9992(n=6);测得2批天麻含片的天麻苷含量分别为1.0520mg/片和0.9692mg/片,平均回收率95.28%;方法精密度(RSD=0.98%,n=5)和重现性(RSD=0.78%,n=5)良好;天麻苷在4h内测定稳定RSD=1.16%。结论:该法用于测定天麻含片中天麻苷的含量,操作简便快速,结果准确可靠。
Objective: To establish a method for the determination of gastrodin content in Tianma Lozenges by high performance liquid chromatography (HPLC) in order to provide a basis for the establishment of quality control standards for Tianma lozenges. Methods: HPLC, gastrodin as a reference substance, Zorbax SB-C18 bonded silica gel column (4.6mm×150mm, 5μm), 0.05% phosphoric acid: acetonitrile (3:97) as mobile phase, flow rate 1.0ml.min-1, The column temperature was room temperature and the detection wavelength was 220 nm. RESULTS: There was a good linear relationship between the range of 0.049~0.396 ml-1 and the peak area of gastrodine, the regression equation A=1.71×106+5.864×107, r=0.9992 (n=6); 2 batches of gastrodia elata were measured. The content of glucosides was 1.0520 mg/tablet and 0.9692 mg/tablet respectively, the average recovery rate was 95.28%; the method precision (RSD=0.98%, n=5) and reproducibility (RSD=0.78%, n=5) were good; The glycosides were determined to have a stable RSD of 1.16% within 4 h. Conclusion: This method is used to determine the content of gastrodine in Tianma Lozenge. The operation is simple and rapid, and the result is accurate and reliable.