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  5. CLONING AND DETERMINING OF BAC GENE AND Bcl-2 AND CDK4 EXPRESSION ON ASCITES HEPATOMA CELL LINE Hca-

CLONING AND DETERMINING OF BAC GENE AND Bcl-2 AND CDK4 EXPRESSION ON ASCITES HEPATOMA CELL LINE Hca-

来源 :中国癌症研究（英文版） | 被引量 : 0次 | 上传用户：dexter001
【摘 要】
：
Objective: To study the mechanism of cancer, the DNA for BAC was cloned from an ascites hepatoma cell line Hca-F25/CL-16A3 using PCR. Methods: The nucleotide se
【作 者】
：
ZUO Yun-fei ZHANG Yao-zheng ZH 
【机 构】
：
Department of Clinic Biochemistry,Department of Traditional Chinese Medicine,Department of Surgery
【出 处】
：
中国癌症研究（英文版）
【发表日期】
：
2004年期
【关键词】
：
BAC gene bcl-2 CDK4 Hca-F25/CL-16A3 
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Objective: To study the mechanism of cancer, the DNA for BAC was cloned from an ascites hepatoma cell line Hca-F25/CL-16A3 using PCR. Methods: The nucleotide sequences were determined using ABI PRISMTM 377 DNA sequencer. The expression of bcl-2 and CDK4gene were determined using immunohistochemistry.Results: The sequences of BAC segment on HcaF25/CL-16A3 have nearly identical sequences with human BAC. The bcl-2 and CDK4 are highly expression on this cell line. Conclusion: The highly expression of bcl-2 and CDK4 may the one of mechanisms for tumor growth.
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