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随着神经化学的发展,使得人们有可能应用某种神经毒素特异性地毁损某些神经元,Ungerstedt首先在大鼠黑质定位注射6-OHDA,成功地制备了Parkison’s病(PD)动物模型,本文就如何进一步提高PD模型成功率进行一些探讨。 材料和方法 选用体重150～250 g的SD大鼠50只,雌雄不拘,45只为实验组,5只为对照组。术前用戊巴比妥钠(30 mg/kg)腹腔麻醉,头部固定于立体定向仪上(江湾Ⅰ型C),手术暴露前后囟,调整颅骨平面,使后囟低于前囟1 mm,同时测量前后囟之间的距离(后囟位置以人字缝尖为准,在实验中测得该间距为5.8～7.5 mm)。选取两个注射点,其坐标参数分别为:后囟向前0.33×前后囟间距
With the development of neurochemistry, it is possible that some neurotoxins may be used to specifically damage certain neurons. Ungerstedt first successfully established Parkinson’s disease (PD) animal model by injecting 6-OHDA into the substantia nigra of rats, This article discusses how to further improve the success rate of PD model. MATERIALS AND METHODS Fifty SD rats weighing 150-250 g were used, both male and female, 45 for the experimental group and 5 for the control group. Preoperative anesthesia with sodium pentobarbital (30 mg / kg), the head fixed in the stereotaxic instrument (Jiang Wan Ⅰ C), before and after surgery exposed skull, adjust the skull plane, the posterior fontanelle less than the anterior fontanelle 1 mm, while measuring the distance between the anterior and posterior fontanelle (posterior fontanel position to the word seam, whichever is the test, measured in the distance of 5.8 ~ 7.5 mm). Select two injection points, the coordinate parameters were: bregma forward 0.33 × anteroposterior distance