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目的 :观察柔红霉素、长春新碱联合应用在体外对人急性髓系白血病细胞株 (HL—60)的相互作用。方法 :采用MTT法 ,利用中效原理判断联合用药的效果。结果 :两种药物单用及联合应用时随着药物剂量增加 ,其效应也增加。两药大剂量合用时为拮抗效应 ,较小剂量合用时为协同效应。两药合用时 (除最小剂量外 )与先用柔红霉素24小时或先用长春新碱24小时比较P<0 01 ;小剂量合用时先用柔红霉素24小时与先用长春新碱24小时比较P>0 05。两药合用时浓度变化会影响合用效应。结论 :柔红霉素与长春新碱合用时大剂量为拮抗 ,较小剂量为协同 ;合用效应大小与两种药物浓度比例有关 ;与给药先后次序无关 ,同时给药效果好
Objective: To observe the interaction between daunorubicin and vincristine in human acute myeloid leukemia cell line (HL-60) in vitro. Methods: MTT method, the use of the principle of effect to determine the effect of combination therapy. Results: The effects of the two drugs increased with the increase of the dose of the drugs when used alone and in combination. Two drugs when combined with high doses for the antagonistic effect, when combined with smaller doses for the synergistic effect. The combination of two drugs (except for the minimum dose) with daunorubicin first 24 hours or first with vincristine 24 hours P <0 01; low-dose combination of daunorubicin first with 24 hours and first with Changchun new Base 24 hours compared P> 0 05. Combination of two drugs when the concentration changes will affect the combined effect. CONCLUSION: The combination of daunorubicin and vincristine is antagonistic at high dose, and the smaller dose is synergistic. The size of combined effect is related to the ratio of two drug concentrations.