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目的 建立采用高效液相色谱分离复方黄连素注射液中的甲氧苄啶和硫酸氢黄连素的方法。方法 色谱柱为KromasilODS(5 μg ,4 6mm× 2 5 0mm) ,流动相为乙腈— 0 0 0 5mol·L- 1 庚烷磺酸钠溶液 (4 0 :60 ,用冰醋酸调节pH至 3 0 ) ,流速1 0mL·min- 1 ,检测波长 2 70nm。结果 线性范围分别是 :甲氧苄啶 7 5 1～ 15 0 12 μg·mL- 1 ,r =0 9999;硫酸氢黄连素 2 5 1～5 0 14 μg·mL- 1 ,r=0 9999。回收率 :甲氧苄 10 0 3 % ;硫酸氢黄连素 99 9%。结论 本方法简便 ,结果准确可靠 ,适用于该制剂两组分的同时测定
Objective To establish a method for the separation of trimethoprim and berberine sulfate from compound berberine injection by high performance liquid chromatography. Methods The chromatographic column was Kromasil ODS (5 μg, 4 6 mm × 250 mm). The mobile phase consisted of acetonitrile- 0 0 0 5 mol · L-1 sodium heptane sulfonate solution (40: 60). The pH was adjusted to 3 0 with glacial acetic acid. ), flow rate 10 mL·min-1, detection wavelength 2 70 nm. The linear ranges of the results were: trimethoprim 75 1 - 15 0 12 μg·mL - 1, r = 09999; berberine sulfate 2 5 1 - 5 0 14 μg·mL - 1, r = 0 9999. Recovery: trimethoprim 10 0 3%; berberine sulfate 99 9%. Conclusion This method is simple, accurate and reliable, suitable for simultaneous determination of two components of the preparation