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An enzyme biosensor for amperometric measurement of aspartate aminotransferase has been developed.The working electrode was modified with a thin-film of redox polymer,then glutamate oxidase,with the immobilized reagent cast and dried on the electrode.The biosensor responses to AST by detecting hydrogen peroxide were produced by enzymical reaction at-0.1 V with a response time of 120 seconds.The electrode gave a detection limit of 32.5 U/L with a linear concentration range of 32.5 U/L～2000 U/L in serum.Due to more sensitive and lower detection limit,the biosensor is expected mainly to be used for physiological identification and physical performance of athletes in the future.Extended application will also affect the practice of clinical medicine for the diagnosis of heart and liver disease.
An enzyme biosensor for amperometric measurement of aspartate aminotransferase has been developed. The working electrode was modified with a thin-film of redox polymer, then glutamate oxidase, with the immobilized reagent cast and dried on the electrode. Biosensor responses to AST by detecting hydrogen Peroxide were produced by an enzymatic reaction at-0.1 V with a response time of 120 seconds. The electrode gave a detection limit of 32.5 U / L with a linear concentration range of 32.5 U / L ~ 2000 U / L in serum. Due to more sensitive and lower detection limit, the biosensor is expected mainly to be used for physiological identification and physical performance of athletes in the future. Extended application will also affect the practice of clinical medicine for the diagnosis of heart and liver disease.