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目的探讨干扰素-γ对实验性大鼠肝组织中的基质金属蛋白酶-13(MMP-13)与金属蛋白酶组织抑制因子-1(TIMP-1)表达的影响。方法SD大鼠22只,随机分为3组:正常组(7只)、模型组(6只)和干扰素-γ组(9只);用四氯化碳制备大鼠肝纤维化模型,在10周末处死各组大鼠,取肝脏进行HE染色,并运用半定量逆转录聚合酶链反应(RT-PCR)检测肝组织中MMP-13 mRNA与TIMP-1 mRNA的表达量。结果MMP-13 mRNA的表达,与正常组对比,模型组和干扰素-γ组的MMP-13 mRNA的表达量增高(P<0.01),但模型组和干扰素-γ组之间还不能认为有统计学差别(P>0.01);而TIMP-1 mRNA表达与正常组相比,模型组和干扰素-γ组中的TIMP-1 mRNA的表达都升高(P<0.01),而且模型组中的TIMP-1 mRNA表达量比干扰素-γ组高(P<0.01)。在肝纤维化病理学观察的量化秩和分析中,各组之间的差别明显(P<0.005)。结论干扰素-γ逆转肝纤维化的机制可能是减少肝纤维化大鼠肝脏中的TIMP-1mR-NA的表达,从而逆转肝纤维化。
Objective To investigate the effect of interferon-γ on the expression of matrix metalloproteinase-13 (MMP-13) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in experimental rat liver. Methods Twenty-two SD rats were randomly divided into three groups: normal group (n = 7), model group (n = 6) and interferon-γ group (n = 9). Rat models of hepatic fibrosis were induced by carbon tetrachloride The rats were sacrificed at the end of the 10th week, and the liver was harvested for HE staining. The expression of MMP-13 mRNA and TIMP-1 mRNA in liver tissues was detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). Results Compared with normal group, the expression of MMP-13 mRNA in model group and interferon-γ group increased (P <0.01), but the expression of MMP-13 mRNA in model group and interferon- (P <0.01). However, the expression of TIMP-1 mRNA in model group and interferon-γ group was significantly higher than that in normal group (P <0.01), and the expression of TIMP-1 mRNA in model group TIMP-1 mRNA expression was higher in interferon-γ group (P <0.01). In the quantitative rank sum analysis of liver fibrosis pathology, there was a significant difference between the groups (P <0.005). Conclusion Interferon-γ may reverse the mechanism of liver fibrosis by reducing the expression of TIMP-1mR-NA in the liver of rats with hepatic fibrosis, thus reversing hepatic fibrosis.