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采用PCR和DNA测序技术对贵州都柳江鲤(Cyprinus carpio)、鲫(Carassius auratus)和草鱼(Ctenopharyngodon idellus)种群的mtDNA控制区序列及遗传多样性进行了研究。获得了都柳江鲤、鲫和草鱼mtDNA控制区长度分别为899~901 bp、787 bp和901~905 bp的序列。该3种鱼类控制区碱基A、T含量较高,G含量最低。识别了该3种鱼类mtDNA控制区终止序列区、中央保守区和保守序列区等保守序列。其中,除CSB-2和CSB-3碱基组成相同外,其余核心序列碱基组成存在着差异。都柳江鲤、鲫和草鱼种群mtDNA控制区分别有24、24和11个多态位点,分属12、17和8个单倍型。都柳江鲤、鲫种群遗传多样性较高,草鱼种群遗传多样性较低。因此,有必要开展都柳江草鱼种群遗传多样性的保护。
The mtDNA control region and genetic diversity of Cyprinus carpio, Carassius auratus and Ctenopharyngodon idellus population were studied by PCR and DNA sequencing. The sequences of 899 ~ 901 bp, 787 bp and 901 ~ 905 bp in the mtDNA control region of the carp, crucian carp and grass carp were obtained. The three kinds of fish control area base A, T content is higher, G content is the lowest. Identification of the three kinds of fish mtDNA control region termination sequence region, the central conserved region and conserved sequence regions and other conserved sequences. Among them, except for the same base composition of CSB-2 and CSB-3, there are differences in base composition of other core sequences. There were 24, 24 and 11 polymorphic sites in the mtDNA control region of the carp, crucian carp and grass carp, respectively, belonging to 12, 17 and 8 haplotypes. Duliujiang carp and crucian carp had higher genetic diversity and lower genetic diversity in grass carp. Therefore, it is necessary to carry out the protection of the genetic diversity of the Duliujiang grass carp population.