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目的 :为培育人源细粒棘球蚴细胞系 (株 ) ,以供用于免疫预防研究。方法 :用外科方法从临床确诊的细粒棘球蚴患者的肝脏中取出包囊 ,以生发层和原头节为培养材料 ,采用RPMI1640、199和改良 DMEM培养液 ,用鼠尾胶原蛋白包被和不包被的培养瓶 ,对其进行初培养 ,并进行对比观察。结果 :以胶原蛋白作为支撑材料 ,应用改良 DMEM培养液培养的人源细粒棘球蚴生发层和原头节效果优于其他 ,已成功地培育了人源细粒棘球蚴细胞系 ,并传至第 2 0代。原代培养时间需 2 8d- 4 5d,3代以内细胞为多形态性。结论 :建立了适合人源细粒棘球蚴细胞体外培养的方法。
OBJECTIVE: To develop human Echinococcus granulosus cell line for use in immunoprophylaxis research. Methods: Surgical methods were used to remove the cysts from the liver of patients with clinically diagnosed Echinococcus granulosus. The germinal layer and procambium were used as the culture materials. RPMI1640 and 199 and modified DMEM medium were used to coat with rat tail collagen And not coated flasks, their initial culture, and comparative observation. Results: Collagen was used as the supporting material, and the cultured echinococcus granulosus and protostems cultured with modified DMEM medium had better effect than the others, and successfully cultured human Echinococcus granulosus cell line Passed on to the 20th generation. Primary culture time required 28d-45d, within 3 generations of cells for multi-morphological. Conclusion: The method of in vitro culture of human Echinococcus granulosus cells was established.