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用放射免疫法（ＲＩＡ）和声谱分析法，先后两次检测了４２５名４～７９岁的健康人和１４９名１３～１９岁的戏曲、声乐学员的血清睾酮（Ｔ）、雌二醇（Ｅ_２）和基频（Ｆ_０）。结果发现：（１）Ｆ_０随年龄增长而下降，男性比女性明显；Ｔ及Ｅ_２则随年龄增长而上升，从１０岁开始男性Ｔ和女性Ｅ_２上升明显，而Ｆ_０也从１０岁开始男女有显著差别；（２）１３～１９岁性激素波动较大，但男性者仍和年龄呈正相关；男女的Ｆ_０皆在这一时期有一个回升又下降的阶段；（３）女性４０岁以后，Ｆ_０及Ｅ_２、Ｔ皆持续下降，Ｅ_２呈陡降形式；男性４０岁以后Ｔ呈陡降形式，但５０岁以后，Ｆ_０及Ｅ_２皆呈上升趋势；（４）影响嗓音变化的因素以Ｔ为显著，但Ｅ_２也不无关系。
By radioimmunoassay (RIA) and spectroscopic analysis, 425 healthy subjects aged from 4 to 79 and 149 male and female vocal music students aged 13 to 19 years were tested for serum testosterone (T), estradiol E_2) and fundamental frequency (F_0). The results showed that: (1) F_0 decreased with age, male was more obvious than female; T and E 2 increased with age, male T and female E 2 increased significantly from the age of 10, while F 0 also significantly increased from the age of 10 (2) The sex hormones fluctuated greatly from 13 to 19 years old, but the males were still positively correlated with the age. The male and female F_0 all had a rising and falling stage in this period. (3) After 40 years of age, , T both continued to decline and E_2 showed a steep decline. After 40 years of age, T showed a steep drop, but F_0 and E_2 showed an upward trend after 50 years of age. (4) The factors that affected the voice change were T, but E_2 Not unrelated.