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目的探讨姜黄素对博莱霉素(BLM)诱导的急性肺损伤(Acute lung injury,ALI)小鼠肺组织谷胱甘肽过氧化物酶(GSH-PX)、诱导型一氧化氮合酶(iNOS)活性的影响。方法博莱霉素诱导小鼠急性肺损伤模型,光镜观察肺组织学改变,测定GSH-PX、iNOS活性变化。结果姜黄素能降低肺组织中iNOS活性,提高小鼠体内GSH-PX活性,与假手术组相比,第3、7天时模型组iNOS活性明显增高(P<0.01),姜黄素中、高剂量组和模型组相比,iNOS活性下降显著(P<0.01);与假手术组相比,第3、7天时模型组GSH-PX活性明显减少(P<0.01),姜黄素中、高剂量组和模型组相比,GSH-PX活性显著增加(P<0.01)。病理组织学检查亦表明,一定剂量的姜黄素可明显减轻肺组织损伤的程度。结论一定剂量姜黄素在BLM诱导的急性肺损伤中有一定的防治作用。其机制可能是通过升高肺组织的GSH-PX活性,降低iNOS的活性,重建机体氧化、抗氧化平衡有关。
Objective To investigate the effect of curcumin on glutathione peroxidase (GSH-PX) and inducible nitric oxide synthase (iNOS) in lung tissue of mice with BLM-induced acute lung injury (ALI) iNOS) activity. Methods Bleomycin induced the acute lung injury model in mice. The lung histological changes were observed with light microscope. The changes of GSH-PX and iNOS activity were measured. Results Curcumin decreased iNOS activity and increased GSH-PX activity in mice. Compared with sham operation group, iNOS activity in model group increased significantly (P <0.01) Compared with the sham-operation group, the activity of GSH-PX in the model group decreased obviously on the 3rd and 7th day (P <0.01), the middle and high dose curcumin group Compared with the model group, GSH-PX activity increased significantly (P <0.01). Histopathological examination also showed that a certain dose of curcumin can significantly reduce the extent of lung injury. Conclusion A certain dose of curcumin has some preventive and therapeutic effects on BLM-induced acute lung injury. The mechanism may be related to elevating the activity of GSH-PX in lung tissue, decreasing the activity of iNOS, and reconstructing the body’s oxidation and antioxidant balance.