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目的检测KISS-1及GPR54mRNA在子宫内膜癌组织中的表达,探讨其在子宫内膜癌浸润及转移中的作用。方法采用逆转录多聚酶链反应(RT-PCR)法检测32例子宫内膜癌,10例子宫内膜上皮内瘤样病变(EIN)及12例正常子宫内膜组织中KiSS-1及GPR54mRNA的表达情况和其与各种临床参数的关系。结果KiSS-1mRNA在子宫内膜癌组中的表达率(37.5%)明显低于EIN组(80.0%)及正常内膜组(83.3%),并且其在子宫内膜癌组中的表达率与临床分期、肌层浸润深度及淋巴结转移密切相关,P均<0.05。GPR54mRNA在子宫内膜癌组的表达率(78.1%)高于EIN组(70.0%)和正常内膜组(66.7%),但无明显差异(均P>0.05)。GPR54mRNA在子宫内膜癌组中的表达与临床分期、组织分级、肌层浸润深度及淋巴结转移情况均无相关性(均P>0.05)。结论KiSS-1与其受体GPR54结合在抑制子宫内膜癌的浸润和转移过程中可能起着重要的作用。
Objective To detect the expression of KISS-1 and GPR54 mRNA in endometrial carcinoma, and to explore its role in the invasion and metastasis of endometrial carcinoma. Methods The expressions of KiSS-1 and GPR54 mRNA in 32 cases of endometrial carcinoma, 10 cases of endometrial intraepithelial neoplasia (EIN) and 12 cases of normal endometrium were detected by reverse transcription-polymerase chain reaction (RT-PCR) The situation and its relationship with various clinical parameters. Results The expression rate of KiSS-1 mRNA in endometrial carcinoma group (37.5%) was significantly lower than that in EIN group (80.0%) and normal endometrium group (83.3%), and the expression rate of KiSS- Clinical stage, myometrial invasion depth and lymph node metastasis were closely related, P <0.05. The expression rate of GPR54 mRNA in endometrial carcinoma group was higher than that in EIN group (70.0%) and normal endometrium group (66.7%) (78.1%), but there was no significant difference (all P> 0.05). The expression of GPR54 mRNA in endometrial carcinoma group had no correlation with clinical stage, histological grade, depth of myometrial invasion and lymph node metastasis (all P> 0.05). Conclusion The combination of KiSS-1 and its receptor GPR54 may play an important role in inhibiting the invasion and metastasis of endometrial carcinoma.