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目的:使用携带红色荧光蛋白(AsRed2)的前列腺癌PC-3细胞(PR7细胞)建立可用活体荧光成像技术监测的前列腺癌肺转移模型。方法:分别采用MTT及Transwell实验比较前列腺癌PC-3与PR7细胞体外增殖、迁移及侵袭能力;将20只BALB/c裸鼠随机平均分为4组,分别尾静脉注射不同浓度PR7细胞悬液200μl,A组:1×107/ml,B组:2.5×107/ml,C组:5×107/ml,D组:2.5×107/ml,并于1周后重复注射2.5×107/ml细胞悬液200μl。第2、4、6、8周分别用活体荧光成像技术检测各组肺转移灶形成情况。结果:前列腺癌PC-3细胞与PR7细胞体外增殖、迁移、侵袭能力均无明显差异(P>0.05);第4周D组40%裸鼠用活体荧光成像检测到肺转移灶;第8周,A组20%、B组60%、C组100%、D组100%裸鼠可检测到肺转移灶;通过病理检查证实肺转移灶形成。结论:通过尾静脉注射携带红色荧光蛋白的前列腺癌PR7细胞可成功建立用活体荧光成像技术监测的前列腺癌肺转移模型。
PURPOSE: To establish a prostate cancer lung metastasis model that can be monitored by in vivo fluorescence imaging using prostate cancer PC-3 cells (PR7 cells) carrying red fluorescent protein (AsRed2). Methods: MTT and Transwell experiments were used to compare the proliferation, migration and invasion ability of PC-3 and PR7 cells in vitro. Twenty BALB / c nude mice were equally divided into 4 groups randomly. The tail vein was injected with different concentrations of PR7 cell suspension 200 μl, group A: 1 × 10 7 / ml, group B: 2.5 × 10 7 / ml, group C: 5 × 10 7 / ml and group D: 2.5 × 10 7 / 200 μl of cell suspension. Fluorescent imaging was used to detect the formation of lung metastases in each group at 2,4,6,8 weeks. RESULTS: There was no significant difference in proliferation, migration and invasion between PC-3 cells and PR7 cells in prostate cancer (P> 0.05). In the fourth week, 40% of nude mice in D group were detected by live fluorescence imaging. , 20% in group A, 60% in group B, 100% in group C, lung metastases were detected in 100% of nude mice in group D; lung metastases were confirmed by pathological examination. CONCLUSIONS: Prostate cancer lung metastasis, which is monitored by live fluorescence imaging, can be successfully established by injecting PR7 cells carrying red fluorescent protein through the tail vein.