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柱状假丝酵母脂肪酶，在异辛烷体系中可选择性地催化Ｓ－２－（６－甲氧基－２萘基）丙酸（萘普森）与三甲基硅甲醇发生酯化反应。利用硅藻土吸附固定化酶有效地改善了酶在有机介质中的分散状态，提高了催化效率。选择和确定了四氯化碳作为辅溶剂——底物酸的增溶剂，并对辅溶剂与异辛烷的比例、醇浓度及温度对酯化反应过程的影响进行了研究。结果表明：在酯化反应中，高浓度的醇具有抑制作用；较高反应温度也导致酶的失活；去除水有利于酯化反应的进行。
Candida cylindracea lipase catalyzes the esterification of S-2- (6-methoxy-2-naphthyl) propionic acid (Naproxen) with trimethylsilyl alcohol in isooctane system . The use of diatomite adsorption immobilized enzyme effectively improve the enzyme in the organic medium dispersion state, improve the catalytic efficiency. The effect of the ratio of auxiliary solvent to isooctane, alcohol concentration and temperature on the esterification reaction process was studied by selecting and determining carbon tetrachloride as the auxiliary solvent - the solubilizer of substrate acid. The results showed that: in the esterification reaction, the high concentration of alcohol inhibition; higher reaction temperature also led to enzyme inactivation; removal of water is conducive to the esterification reaction.