论文部分内容阅读
目的观察不同时程吗啡依赖对大鼠中脑黑质(Substantia nigra,SN)多巴胺能神经细胞的影响。方法按逐日递增原则背部皮下注射盐酸吗啡,建立吗啡依赖大鼠模型,取脑组织经10%福尔马林液固定后石蜡包埋,组织连续切片,免疫组织化学和免疫荧光染色,观察多巴胺能神经细胞特异性标记物酪氨酸羟化酶(tyrosine hydroxylase,TH)蛋白表达变化;Fluoro-Jade B染色观察吗啡依赖不同时程黑质多巴胺能神经细胞变性坏死情况。结果 TH免疫组化结果显示随着吗啡依赖时间的延长黑质阳性细胞逐渐减少,吗啡依赖6周组阳性细胞减少明显。Fluoro-Jade B染色结果证实多巴胺能神经细胞发生了变性坏死。结论较长时程吗啡依赖大鼠黑质多巴胺能神经细胞损伤明显。
Objective To observe the effects of morphine dependence at different time points on dopaminergic neurons in substantia nigra of rats. Methods Morphine hydrochloride was injected subcutaneously on the back of the rats on a daily basis. Morphine-dependent rat model was established. The brain tissue was fixed in 10% formalin solution and then embedded in paraffin. Serial sections, immunohistochemistry and immunofluorescence staining were used to observe the dopaminergic Neuronal cell-specific tyrosine hydroxylase (TH) protein expression was detected by Fluoro-Jade B staining. Morphine dependence on degeneration and necrosis of dopaminergic neurons in substantia nigra at different time points was observed. Results The results of TH immunohistochemistry showed that the number of substantia nigra positive cells gradually decreased with the morphine dependent time prolonging, and the positive cells decreased significantly in morphine dependent 6 week group. Fluoro-Jade B staining confirmed degeneration and necrosis of dopaminergic neurons. Conclusion Long-term morphine-dependent dopaminergic neuronal damage in the substantia nigra is evident.