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目的:探讨试用Earle’s和HTF两种培养液对胚胎质量及体外受精(IVF)妊娠结局的影响。方法:对1999年8月～2001年8月间行试管婴儿(IVF—ET)的患者其中33例共363个卵子分别使用Earie’s培养液(23例23个周期)与使用HTF培养液(10例10个周期)两组进行培养,分析受精率、卵裂率、优质胚胎率及妊娠率,并进行统计学分析。结果:使用HTF培养液组的优质胚胎率、胚胎着床率及妊娠率分别为36.9％、19.6％、60.0％,使用Earle’s培养液组分别为22.3％、3.4％、13.0％,两组比较有非常显著性差异(P<0.01)。结论:采用HTF培养液培养更适合卵子及胚胎的体外发育,从而提高了胚胎质量及IVF的妊娠率。
OBJECTIVE: To investigate the effects of two ear cultures of Earle’s and HTF on embryo quality and pregnancy outcome of in vitro fertilization (IVF). METHODS: A total of 363 eggs from 33 patients (IVF-ET) who underwent IVF-ET between August 1999 and August 2001 were treated with Earie’s medium (23 cases of 23 cycles) and HTF medium (10 cases 10 cycles). The fertilization rate, cleavage rate, high quality embryo rate and pregnancy rate were analyzed and statistically analyzed. Results: The excellent embryo rate, embryo implantation rate and pregnancy rate were 36.9%, 19.6% and 60.0% respectively in the HTF group and 22.3%, 3.4% and 13.0% in the Earle’s medium group respectively Very significant difference (P <0.01). Conclusion: The culture of HTF is more suitable for in vitro development of ovum and embryo, which improves the quality of embryo and the pregnancy rate of IVF.