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从4对已报道的李属树种S-RNase基因引物组合中筛选出扩增效果较好的PruC2和PruC4R组合,对新疆野生樱桃李(Prunus cerasifera Ehrh.)的45个株系的基因组DNA进行S-RNase基因特异性PCR扩增,并对其PCR产物进行克隆测序。这些核酸序列及其相应的氨基酸序列在GenBank中进行比对,皆与李属的S-RNase基因有最大同源性,为新疆野生樱桃李的4种S-RNase基因,分别命名为S1(511bp)、S2(787bp)、S3(1859bp)和S4(464bp),在GenBank的登录号分别为EF638726、EF641276、EF661873和EF661874。45个株系中,43个株系的S基因型分别为S1S2、S1S3、S2S3、S2S4和S3S4,而10号和15号株系分别只鉴定了1种S-RNase基因,其S基因型组成有待于进一步研究。
Four combinations of PruC2 and PruC4R with good amplification were screened from four pairs of reported primer combinations of Prunus cerasifera Ehrh. The genomic DNA of 45 strains of Prunus cerasifera Ehrh. -RNase gene-specific PCR amplification, and its PCR products were cloned and sequenced. These nucleic acid sequences and their corresponding amino acid sequences were aligned in GenBank and all had the greatest homology with the S-RNase gene of Prunus. They were four S-RNase genes of Xinjiang wild cherry plum and were named as S1 (511 bp ), S2 (787bp), S3 (1859bp) and S4 (464bp). The S genotypes of 43 lines were S1S2 in GenBank accession numbers EF638726, EF641276, EF661873 and EF661874.4. S1S3, S2S3, S2S4 and S3S4. However, only one S-RNase gene was identified in lines 10 and 15, respectively. The S genotype of the S-RNase gene remains to be further studied.