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目的：构建KDR基因重组的T7噬菌体疫苗以及探讨其对小鼠Lewis肺癌的抗肿瘤作用。方法：克隆KDR的膜外Ⅱ区基因,构建KDR基因重组T7噬菌体疫苗,免疫小鼠,免疫四次后接种Lewis肺癌,观察肿瘤的生长情况,14 d后脱颈椎杀死小鼠,眼球取血、取出瘤体称重,计算抑瘤率,观察抗肿瘤效果。ELISA检测小鼠血清抗KDR抗体滴度。结果：实验组抑制程度明显,肿瘤生长速度最慢,肿瘤重量与生理盐水组和空白噬菌体组相比有统计学差异(P＜0．05),抑瘤率可达57．0%,远大于空白噬菌体组(16．0%)。小鼠血清稀释至1∶500,小鼠特异性抗人源KDR抗体仍呈阳性。结论：KDR基因重组的T7噬菌体疫苗对小鼠Lewis肺癌具有明显的抗肿瘤作用,用人源KDR作为免疫原免疫小鼠通过免疫交叉反应可以打破对自身抗原的免疫耐受,产生特异性的免疫反应。
OBJECTIVE: To construct recombinant T7 phage vaccine with KDR gene and explore its antitumor effect on Lewis lung carcinoma in mice. Methods: The gene fragment Ⅱ of KDR was cloned and the KDR gene recombinant T7 phage vaccine was constructed. The mice were immunized four times and then immunized with Lewis lung carcinoma for 4 days. The growth of the tumor was observed. After 14 days, the mice were sacrificed by cervical dislocation, , Remove the tumor weight, calculate the inhibition rate, observe the anti-tumor effect. ELISA test serum anti-KDR antibody titers. Results: The experimental group had obvious inhibition, the slowest tumor growth rate, the tumor weight compared with the saline group and the blank phage group (P <0.05), and the tumor inhibition rate was 57.0% Blank phage group (16.0%). Mouse serum was diluted to 1: 500 and mouse-specific anti-human KDR antibodies were still positive. CONCLUSION: KDR gene recombinant T7 phage vaccine has obvious anti-tumor effect on Lewis lung cancer in mice. Immunization of mice with human KDR as immunogen can break the immune tolerance to autoantigen through immune cross-reaction and produce specific immune response .