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目的探讨GluR5、GluR6在马桑内酯与ATPA致痫的大鼠星形胶质细胞中的不同表达及其在致痫模型中可能的作用机制。方法采用ATPA和马桑内酯(coriaria lactone,CL)分别诱导处理大鼠星形胶质细胞,加入等量生理盐水培养的星形胶质细胞作为对照组,通过RT-PCR和Western blot检测细胞中GluR5、GluR6的mRNA和蛋白质水平。结果 ATPA作用后星形胶质细胞GluR5的mRNA和蛋白质表达较对照组升高(P<0.05),GluR6的表达较对照组无明显变化(P>0.05);CL作用后星形胶质细胞GluR5和GluR6表达均明显降低(P<0.05)。结论 ATPA上调星形胶质细胞的GluR5受体,而CL下调星形胶质细胞的GluR5、GluR6受体,提示GluR5、GluR6在不同致痫剂诱导的癫痫活动中有不同的作用机制,CL对GluR5和GluR6可能存在负反馈调节,具体分子机制尚需进一步研究。
Objective To investigate the different expression of GluR5 and GluR6 in rat astrocytes induced by docetaxel and ATPA and its possible mechanism in epileptic model. Methods Rat astrocytes were induced by ATPA and coriaria lactone (CL), and astrocytes cultured in the same amount of saline were added as control group, and the cells were detected by RT-PCR and Western blot GluR5, GluR6 in mRNA and protein levels. Results The mRNA and protein expression of GluR5 in astrocytes increased (P <0.05) and the expression of GluR6 in astrocytes was not significantly different from that in control group (P> 0.05) And GluR6 expression were significantly lower (P <0.05). Conclusion ATPA upregulates GluR5 receptor in astrocytes, while CL downregulates GluR5 and GluR6 receptors in astrocytes, suggesting that GluR5 and GluR6 have different mechanisms of action in different epilepsy-induced seizures. CL GluR5 and GluR6 may exist negative feedback regulation, the specific molecular mechanism needs further study.