Objective: This study aims to screen the differentially expressed genes of glioblastoma using microarray technique. Methods: Specimens of glioblastoma and normal brain tissue were obtained from pathologically confirmed patients.A cDNA microarray comprising 14 000 clones covering the whole sets of the retro-transcriptional products of the mRNAs of various gliomas and those of normal brain tissues was established, with which the differences in gene expression between glioblastoma and normal brain tissues were investigated. Results: It was found that 94 genes were more than 3-fold differentially expressed with 298 more than doubled in the glioblastoma in comparison with the normal brain tissue. Some over-expressed genes in the glioblastoma were scarcely expressed in normal brain tissues, and several novel genes that may have biological relevance in the process ofglioma genesis were identified. Conclusion: Microarray technique combined with relevant cDNA repository can facilitate rapid large-scale identification of potential target genes for diagnosis and.therapy of glioma.