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目的观察体外单用及两者联合应用半胱天冬酶原活化复合物-1(PAC-1)和奥沙利铂(L-OHP)对人结肠腺癌细胞株HCT116、SW480、HT-29,SW116和SW620的生长抑制作用。方法 MTT法检测两药单用和合用前后对细胞增殖活性的影响。结果 PAC-1和L-OHP单用对5种人结肠癌细胞均有明显的诱导凋亡和增殖抑制作用,并呈时间和浓度依赖性;PAC-1对上述细胞的半数抑制浓度(IC50)之间无统计学差异(P>0.05),L-OHP对上述细胞的的IC50值有统计学意义(P<0.05)。PAC-1与L-OHP联用(同时给药)产生拮抗作用(CI(1)。结论 PAC-1与L-OHP单用均对人结肠腺癌细胞有抑制作用,但各细胞株对两药的敏感性不同,两者联合应用(同时给药)起拮抗作用。
Objective To observe the effects of combination of PAC-1 and L-OHP on human colon adenocarcinoma cell lines HCT116, SW480, HT-29 , SW116 and SW620 growth inhibitory effect. Methods MTT assay was used to determine the effect of two drugs on cell proliferation activity before and after treatment. Results Both PAC-1 and L-OHP alone induced significant apoptosis and proliferation inhibition in 5 kinds of human colon cancer cells in a time and concentration-dependent manner. The IC50 values of PAC- (P> 0.05). The IC50 values of L-OHP in these cells were statistically significant (P <0.05). Antagonistic effect of PAC-1 and L-OHP combined with simultaneous administration (CI (1) .CONCLUSION PAC-1 and L-OHP alone can inhibit human colon adenocarcinoma cells, The sensitivity of the drug is different, the two combined (simultaneous administration) from the antagonistic effect.