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目的探讨雄激素受体(AR)基因CAG、GGN重复片段多态性与指长比之间的关联性。方法研究对象来自宁夏医科大学2011级健康学生共685例,男性294例,平均年龄(20.02±1.28)岁,女性391例,平均年龄(19.25±1.55)岁。用ABI 3730XL测序仪测定AR基因的(CAG)n及(GGN)n重复数目,照片打好点后用电子游标卡尺对指长比进行测定。2D∶4D经均值±2标准差筛选,用独立样本t检验检测2D∶4D在男性与女性个体中的分布差异。用四分位法筛选出较低(Q1)与较高(Q3)2D∶4D。用相关分析检验AR基因中CAG和GGN两个多态性位点与2D∶4D的关联性。结果女性右手、左手及左右手平均2D∶4D均高于男性(P<0.05,P<0.01,P<0.01),但DR-L2D∶4D的分布差异无统计学意义(P>0.05)。AR CAG/GGN重复多态性与男性右手及左手2D∶4D无关联性(P>0.05),但CAG重复多态性与Q1 DR-L及Q3平均2D∶4D存在关联性(r=0.280,P<0.05,r=0.274,P<0.05)。女性较短CAG等位基因与Q3平均2D∶4D存在关联性(r=0.337,P<0.05),女性较长CAG等位基因与Q3右手及DR-L2D∶4D存在关联性(r=0.238,P<0.05;r=0.175,P<0.05),女性平均CAG等位基因与Q3平均2D∶4D存在关联性(r=0.236,P<0.05)。女性较长GGN等位基因与女性右手2D∶4D存在关联性(r=0.204,P<0.05),女性平均GGN等位基因与Q3平均2D∶4D存在关联性(r=0.225,P<0.05)。结论 AR基因CAG与GGN重复片段多态性可能与指长比存在关联性,联合运用四分位法与相关分析可能是揭示AR多态性与指长比关联性的一种更好的方法。
Objective To investigate the association between the polymorphism of CAG and GGN repeats in the androgen receptor (AR) gene and the odds ratio. Methods A total of 685 grade 2011 healthy students from Ningxia Medical University were enrolled in this study. There were 294 males, average age (20.02 ± 1.28) years old and 391 females with average age (19.25 ± 1.55) years old. The AR gene (CAG) n and (GGN) n repeats were determined by the ABI 3730XL sequencer. After the photos were taken, the fin ratio was measured by electronic vernier calipers. 2D: 4D was screened by mean ± 2 standard deviation, and the independent sample t-test was used to examine the distribution of 2D: 4D in male and female individuals. The lower (Q1) and higher (Q3) 2D: 4D were screened by quartiles. Correlation analysis was used to examine the association of two polymorphic sites of CAG and GGN in AR gene with 2D: 4D. Results The average 2D: 4D of right hand, left hand and right hand were higher than those of male (P <0.05, P <0.01, P <0.01). However, the distribution of DR-L2D: 4D was not statistically different (P> 0.05). AR CAG / GGN polymorphism was not associated with right-handed and left-handed 2D:4D in men (P> 0.05), but there was a correlation between CAG repeat polymorphism and Q1 DR-L and Q3 average 2D:4D (r = 0.280, P <0.05, r = 0.274, P <0.05). The association of shorter CAG alleles in women with Q3 mean 2D: 4D (r = 0.337, P <0.05) and longer female CAG alleles with Q3 right and DR-L2D: P <0.05; r = 0.175, P <0.05). The average female CAG allele was correlated with Q3 2D: 4D (r = 0.236, P <0.05). There was a correlation between the longer GGN allele in women and the right 2D: 4D in women (r = 0.204, P <0.05). The average GGN allele in female was correlated with 2D 2DD in Q3 (r = 0.225, . Conclusions ARGC polymorphism of CAG and GGN fragments may be related to the ratio of length to length. Using a combination of quartiles and correlation analysis may be a better way to reveal the association between AR polymorphism and odds ratio.