翘鳞肉齿菌菌丝液体培养是工厂化生产菌丝的基础，液体培养试验分别在培养液配方、装液量、培养时间及振荡转速等方面进行了研究．试验结果表明，翘鳞肉齿菌菌丝在ＰＤ和ＹＭ培养液中生长良好，在５００ｍｌ三角瓶中装培养液１４０ｍｌ，培养５ｄ，振荡转速在１００ｒ／ｍｉｎ条件下，菌丝得率最高，可达到０．６％～０．７％． The mycelial growth of Mycobacterium longiflorus was the basis of mycelial production in laboratory. The liquid culture experiments were carried out in the aspects of culture solution, liquid volume, culture time and shaking speed. The results showed that the mycelium growth of M. castaneum in PD and YM medium was good. In 500 ml flask, 140 ml culture medium was cultured for 5 days. Under the conditions of 100r / min shaking speed, the yield of mycelia was the highest, Reached 0.6% ~ 0.7%.