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[目的]探讨鳖卵污染O139/O1霍乱弧菌后的带菌时间。[方法]采用菌液浸泡种卵后人工孵化,及菌液与沙混合后埋入卵直接孵化2种方法。孵化期间对沙、卵外壳、卵内容物,及稚鳖、养鳖用水进行O139/O1VC检测。[结果]在孵化期(39d)内,O139VC浸泡卵基本阳性(仅沙和卵壳各1次阴性),卵内容物多数阳性(8/14);O1VC浸泡卵上述3项均阳性(8/8)。染菌湿沙孵化的种卵,沙及卵外壳孵化期阳性检出时间较浸泡组短,仅1枚卵内容物阳性,其它检次均阴性。从第1只稚鳖孵出的3～56d,检鳖10只(包括对照2只),检水8次,均未检出O139/O1VC。[结论]如不考虑鳖卵在沙中的重复感染因素,卵外壳和卵内O139/O1VC的带菌时间至少可达39d,但未见延续至稚鳖出壳后。
[Objective] The aim of this study was to investigate the bacteriocidal time of turtle eggs infected with Vibrio cholerae O139 / O1. [Method] Two methods were used to hatch the eggs and soak them into the eggs and incubate them directly. O139 / O1VC detection of sand, egg shell, egg contents, and juvenile soft-shelled turtle and turtle with water during hatching. [Results] In the incubation period (39 days), the soaked eggs of O139VC were basically positive (only negative in the sand and eggshell, negative in the eggshell), and most of the eggs were positive (8/14) 8). The positive detection time of hatching stage of eggs, sand and egg shell of hatching bacteria wet sand was shorter than that of soaking group, and only one egg content was positive, while the others were negative. From the first juvenile turtle hatch 3 ~ 56d, seized turtle 10 (including the control 2), seized water 8 times, were not detected O139 / O1VC. [Conclusion] If we do not consider the repeated infection of turtle eggs in sand, the carrying time of O139 / O1VC in eggshell and ova could reach at least 39 days, but it did not extend to hatching.