肿瘤血管结构不同于正常组织血管,由内皮细胞松散地围成不完整的管道,呈高通透性。为建立体外简便、有效、低廉的肿瘤血管模型,本研究采用微加工技术,制作了肿瘤血管微流控芯片。芯片包括24 mm长50μm宽40μm深的微管道和一侧的微腔体以及两者之间的微缝。微管道中部设有膨胀区域以模拟肿瘤血管结构。在微管道内导入血管内皮细胞,培养形成细胞壁垒。通过促进细胞迁移而使得细胞壁垒出现稳定的泄漏点,使血管芯片具有通透性,并对体内调控血管通透性分子组胺和MMP2产生与体内相似的反应。在此基础上发现,原花青素对血管通透性有显著抑制作用,而抗癌药物依立替康则有促进作用,显示该肿瘤血管微流控芯片初步具备了肿瘤血管通透性调控分析的功能。 Tumor vascular structure is different from normal tissue blood vessels, loosely surrounded by endothelial cells into an incomplete pipeline, was highly permeable. In order to establish a simple, effective and inexpensive tumor vascular model in vitro, this study used micro-processing technology to produce tumor vascular microfluidic chip. The chip consists of a 24 mm long microchannel with a width of 50 μm and a width of 40 μm and a microcavity on one side and a slight gap between the two. The central part of the microchannel is provided with an expansion area to mimic the tumor vascular structure. Vascular endothelial cells are introduced into the microchannels and cultured to form cell wall barriers. By promoting cell migration and cell barriers appear stable leakage point, the vascular chip permeability and histamine and MMP2 regulate the vascular permeability molecules in vivo produce a similar response in vivo. On this basis, it was found that proanthocyanidins have a significant inhibitory effect on vascular permeability, while the anti-cancer drug irinotecan has a promoting effect, indicating that the tumor vascular microfluidic chip initially has the function of tumor vascular permeability regulation analysis.