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目的:观察复方中药组分(黄连生物碱40%、三七总皂苷35%、麦冬多糖25%)对糖尿病模型大鼠血浆内皮素-1(ET-1)、血管紧张素Ⅰ(AngⅠ)、血管紧张素Ⅱ(AngⅡ)的影响。方法:用链脲菌素制备糖尿病大鼠模型,随机分为5组(模型对照组、二甲双胍组及高、中、低剂量中药组),每组各10只;另取10只作为空白对照组。其中模型对照组和空白对照组用0.9%Na Cl溶液10 ml·kg-1·d-1,二甲双胍组用二甲双胍0.05 mg·kg-1·d-1,高、中、低剂量中药组分别用中药组分0.45、0.15、0.05 g·kg-1·d-1,连续灌胃干预4周后断头采血;用酶联免疫吸附法检测血浆中ET-1、AngⅠ、AngⅡ的含量。结果:模型对照组ET-1、AngⅠ、AngⅡ含量明显高于空白对照组,差异均有统计学意义(P<0.05,P<0.01)。中、高剂量中药组和二甲双胍组ET-1、AngⅠ含量低于模型对照组,差异有统计学意义(P<0.05);高剂量中药组与二甲双胍组AngⅡ含量低于模型对照组,差异有统计学意义(P<0.05)。高剂量中药组AngⅡ含量低于二甲双胍组,差异有统计学意义(P<0.05)。结论:复方中药组分可能通过降低ET-1、AngⅠ和AngⅡ的水平减轻血管内皮损伤,起到改善糖尿病血管并发症的作用。
OBJECTIVE: To observe the effects of traditional Chinese medicine (40% of Coptis alkaloids, 35% of Panax notoginseng saponins and 25% of polysaccharides of Ophiopogon japonicus) on plasma endothelin-1 (ET-1), angiotensin Ⅰ (AngⅠ) , Angiotensin Ⅱ (Ang Ⅱ). Methods: Diabetic rats were induced with streptozotocin and randomly divided into 5 groups (model control group, metformin group, and high, medium and low dose traditional Chinese medicine groups), each with 10 rats in each group. Another 10 rabbits were used as blank control group . The model control group and blank control group were treated with 0.9% NaCl solution 10 ml · kg-1 · d-1, metformin group with metformin 0.05 mg · kg-1 · d-1, The components of TCM were 0.45,0.15,0.05 g · kg-1 · d-1, and the blood samples were decapitated after continuous intragastric administration for 4 weeks. The contents of ET-1, AngⅠ and AngⅡ in plasma were detected by enzyme-linked immunosorbent assay. Results: The levels of ET-1, AngⅠ and AngⅡ in the model control group were significantly higher than those in the blank control group (P <0.05, P <0.01). The levels of ET-1 and AngⅠ in middle-dose and high-dose traditional Chinese medicine groups and metformin group were lower than those in model control group (P <0.05), while AngⅡ in high-dose traditional Chinese medicine group and metformin group was lower than that in model control group Significance (P <0.05). The content of AngⅡ in high-dose traditional Chinese medicine group was lower than that in metformin group, the difference was statistically significant (P <0.05). CONCLUSION: Compound Chinese herbal medicine may reduce vascular endothelial injury by decreasing the levels of ET-1, AngⅠand AngⅡ, and play an important role in the improvement of diabetic vascular complications.