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[目的]探讨黄荆子乙酸乙酯提取物(EVn-50)体外诱导人宫颈癌Hela细胞的凋亡作用。[方法]体外培养Hela细胞,不同浓度EVn-50(1.0、10.0、100.0μg/ml)作用于Hela细胞。采用PI染色FCM检测EVn-50诱导Hela细胞的凋亡作用;采用间接免疫荧光标记FCM检测凋亡相关蛋白Bcl-2、Bax、Caspase-3的表达。[结果]EVn-501.0、10.0、100.0μg/ml处理Hela细胞48h后,细胞的凋亡率分别为1.06%、8.80%及20.90%,呈浓度依赖性(P<0.05)。不同浓度EVn-50作用Hela细胞48h,细胞中Bax、Caspase-3蛋白表达逐渐升高,Bcl-2蛋白表达逐渐降低(P<0.05)。[结论]EVn-50具有诱导Hela细胞凋亡作用,其作用可能与改变凋亡相关蛋白Bax、Caspase-3及Bcl-2的表达有关。
[Objective] To investigate the apoptosis of human cervical carcinoma Hela cells induced by ethyl acetate extract (EVn-50) of Vitex negundo in vitro. [Method] Hela cells were cultured in vitro. Hela cells were treated with different concentrations of EVn-50 (1.0,10.0,100.0μg / ml). PI staining FCM was used to detect the apoptosis of Hela cells induced by EVn-50. The expressions of Bcl-2, Bax and Caspase-3 were detected by indirect immunofluorescence staining with FCM. [Results] The apoptosis rates of Hela cells treated with EVn-501.0,10.0,100.0μg / ml for 48h were 1.06%, 8.80% and 20.90%, respectively, in a concentration-dependent manner (P <0.05). After treated with different concentrations of EVn-50 for 48h, the expression of Bax and Caspase-3 protein gradually increased and the expression of Bcl-2 protein gradually decreased (P <0.05). [Conclusion] EVn-50 can induce the apoptosis of Hela cells, which may be related to the change of the expression of Bax, Caspase-3 and Bcl-2.