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目的探讨卵巢上皮性肿瘤黑色素瘤细胞黏附因子(melanoma cell adhesion molecule,M-CAM)的表达与微血管密度(microvessel density,MVD)和临床病理特征之间的关系。方法 2007年1月至2008年1月在重庆医科大学附属第一医院应用半定量逆转录聚合酶链反应(RT-PCR)、Western blot、免疫组化检测8例正常卵巢组织、16例卵巢良性上皮性肿瘤(以下简称卵巢良性肿瘤)、40例卵巢上皮性癌(以下简称卵巢癌)组织及20例大网膜转移灶中M-CAM的表达。用CD34标记免疫组化法检测MVD。结果 M-CAM mRNA在卵巢癌细胞可见明显扩增条带,M-CAM mRNA表达值在癌组织和大网膜转移灶中高于卵巢良性肿瘤和正常卵巢组织(P<0.05)。Westernblot显示M-CAM蛋白在正常卵巢组织、卵巢良性肿瘤、卵巢癌组织、大网膜转移灶中M-CAM蛋白的表达值分别为0.314±0.162、0.349±0.135、0.603±0.093、0.636±0.102。免疫组化显示卵巢癌组织的MVD为42.53±8.15,卵巢良性肿瘤的MVD值为20.42±6.07,两者差异有统计学意义(P<0.05)。卵巢癌组织中M-CAM阳性表达组的MVD高于M-CAM阴性表达组。M-CAM蛋白高表达与卵巢癌临床分期、淋巴结转移有关,而与年龄、病理类型、有无腹水、CA125水平等无关。结论 M-CAM的表达不仅与卵巢肿瘤的良、恶性有关,而且与卵巢癌的进展、转移有关;与卵巢癌血管形成有关。
Objective To investigate the relationship between the expression of melanoma cell adhesion molecule (M-CAM) and the microvessel density (MVD) and clinicopathological features in epithelial ovarian cancer. Methods From January 2007 to January 2008, 8 cases of normal ovarian tissue and 16 cases of benign ovarian tissue were examined by RT-PCR, Western blot and immunohistochemistry in the First Affiliated Hospital of Chongqing Medical University. Epithelial tumors (hereinafter referred to as benign ovarian tumors), 40 cases of ovarian epithelial carcinoma (hereinafter referred to as ovarian cancer) tissue and 20 cases of omentum in M-CAM expression. MVD was detected by CD34 labeling immunohistochemistry. Results M-CAM mRNA showed obvious amplification bands in ovarian cancer cells. The expression of M-CAM mRNA was higher than that in benign ovarian tumor and normal ovarian tissue (P <0.05) in cancer tissues and omentum metastasis. Western blot showed that M-CAM protein expression in normal ovarian tissue, benign ovarian tumor, ovarian cancer and omental metastasis were 0.314 ± 0.162,0.349 ± 0.135,0.603 ± 0.093,0.636 ± 0.102 respectively. Immunohistochemistry showed that the MVD was 42.53 ± 8.15 in ovarian cancer tissues and 20.42 ± 6.07 in benign ovarian tumors, the difference was statistically significant (P <0.05). MVD in M-CAM positive cells in ovarian cancer tissues was higher than that in M-CAM negative cells. M-CAM protein overexpression and clinical stage of ovarian cancer, lymph node metastasis, but not with age, pathological type, with or without ascites, CA125 levels and so on. Conclusions The expression of M-CAM is not only related to benign and malignant ovarian tumors, but also to the progress and metastasis of ovarian cancer. It is related to the angiogenesis of ovarian cancer.