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引言用植物血凝素(PHA)作皮肤试验可诱发迟发性超敏反应,无需致敏。尽管其机理还不十分清楚,但癌症病人的PHA反应情况与其预后有关,一般是阳性反应的预后较好,反之较差[1]。在体外PHA是一种致淋巴细胞转化的因子,特别是T淋巴细胞比较敏感。用PHA刺激的体外淋巴细胞转化可作为一个测定T细胞功能的指标[2]。肿瘤病人淋巴细胞的转化率低于对照组的转化率。上述PHA激发的都只是淋巴细胞一般的反应,表明淋巴细胞功能的一个方面,而直接对肿瘤细胞的杀伤作用则更有理论和实际的意义。本实验用微量细胞毒试验来测定肝癌病人和正常人的淋巴细胞在PHA作用下对体外传代的人肝癌细胞系的杀伤力。
Introduction The use of phytohemagglutinin (PHA) as a skin test can induce delayed hypersensitivity without sensitization. Although the mechanism is not yet clear, the PHA response of cancer patients is related to their prognosis. Generally, the positive reaction has a better prognosis, and vice versa. [1] In vitro PHA is a factor that causes lymphocyte transformation, and in particular T lymphocytes are more sensitive. In vitro lymphocyte transformation stimulated with PHA can be used as an indicator of T cell function [2]. The conversion rate of lymphocytes in tumor patients was lower than that in the control group. The above PHA elicited only the general reaction of lymphocytes, indicating one aspect of lymphocyte function, and the direct killing effect on tumor cells is more theoretical and practical. In this experiment, micro-cytotoxicity assay was used to determine the lethality of lymphocytes of liver cancer patients and normal people under the action of PHA on human hepatocellular carcinoma cell lines passaged in vitro.