端粒是真核线性染色体末端结构,人端粒DNA由(TTAGGG)n重复序列组成.端粒酶是核糖核酸蛋白酶,具有RNA依赖的DNA合成酶活性,可重新合成端粒的重复序列.最近研究证实端粒及端粒酶与细胞衰老及肿瘤相关,是近年来生物学研究的热点.检测端粒酶活性是研究端粒酶的基本方法,同时具有潜在的临床诊断价值.本文拟应用最新的端粒重复序列扩增法(Telomeric Repeat AmplificationProtocal,TRAP),检测肝癌细胞端粒酶活性. Telomere is the eukaryotic linear chromosome end structure, human telomeric DNA consists of (TTAGGG) n repeats. Telomerase is a ribonuclease with RNA-dependent DNA synthase activity that allows for the recombination of telomere repeats. Studies have confirmed that telomere and telomerase are related to cell senescence and tumor, which are hot topics in biology research in recent years.Detecting telomerase activity is the basic method for the study of telomerase, and has the potential clinical diagnostic value at the same time.This paper intends to apply the latest Telomeric Repeat Amplification Protocal (TRAP) was used to detect telomerase activity in hepatoma cells.