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乙型肝炎病毒(HBV)和丙型肝炎病毒(HCV)感染肝细胞之后,首先要借助肝细胞中的一些成分完成其复制和表达的生活周期,主要是肝细胞中的一些调节蛋白与这2种肝炎病毒调节基因序列之间的结合,并对于肝炎病毒的复制和表达过程进行调节,肝细胞中一些特有的调节蛋白在肝炎病毒的生活周期中具有十分重要的调节作用,这也是肝炎病毒相对嗜肝细胞特性的分子生物学基础.同样,肝炎病毒的蛋白,或者通过与肝细胞蛋白之间的直接结合,或者通过与DNA的结合以及肝细胞的信号转导途径影响肝细胞的基因表达类型和基因表达水平,或者改变肝细胞基因表达谱的时空规律,影响肝细胞的细胞周期、细胞凋亡、生长分化、信号转导、恶性转化等过程.这是HBV和HCV感染肝细胞之后引起慢性肝脏疾病的重要的分子生物学基础,一直是人们关注的焦点. 中国人民解放军第302医院传染病研究所基因治疗研究中心,在成军博士、教授、主任医师、博士生导师的领导下,建立了筛选肝炎病毒调节基因结合蛋白的酵母单杂交技术,筛选肝炎病毒蛋白、肝细胞蛋白的结合蛋白的酵母双杂交技术,筛选肝炎病毒蛋白反式调节肝细胞基因的抑制性消减杂交和表达谱基因芯片研究技术等,系统研究肝炎病毒与肝细胞相互作用的分子生物学机制,并有新的发现.本专题研究中所发表的这些重要文章,对于进一步深入探索和研究肝炎病毒与肝细胞之间相互作用的分子生物学机制,将产生重要推动作用.
Hepatitis B virus (HBV) and hepatitis C virus (HCV) infection of liver cells, the first to take advantage of some components of liver cells to complete its replication and expression of life cycle, mainly in liver cells, some regulatory proteins and these 2 Hepatitis B virus regulates the binding between genes and regulates the replication and expression of hepatitis B virus. Some unique regulatory proteins in hepatocytes play an important regulatory role in the life cycle of the hepatitis virus, and this is also the case for the hepatitis virus The molecular biology of hepatophilic cell characteristics Similarly, the hepatitis virus protein, either through direct binding to hepatocyte proteins or through DNA binding and hepatocyte signaling pathways, affects the type of hepatocyte gene expression And gene expression, or change the temporal and spatial rules of hepatocyte gene expression profiling, affecting the cell cycle, apoptosis, growth and differentiation, signal transduction, malignant transformation of hepatocytes, which is caused by the chronic hepatitis The important molecular biology of liver disease has always been the focus of attention.Chinese People’s Liberation Army No. 302 Hospital Under the leadership of Dr. Cheng Jun-jun, professor, chief physician and doctoral supervisor, Institute of Infectious Diseases Gene Therapy Center established a yeast single-hybridization screening technology for hepatitis B virus-mediated gene binding protein to screen for the binding of hepatitis virus protein and hepatocyte protein Protein yeast two-hybrid technology, screening of hepatitis virus protein trans-regulation of hepatocyte genes by suppression subtractive hybridization and gene expression profiling gene chip technology, the systematic study of hepatitis B virus and hepatocyte interaction molecular biology mechanism, and a new It is found that these important articles published in this study will play an important role in further exploring and studying the molecular biological mechanism of the interaction between hepatitis virus and hepatocytes.