Objective: To investigate the relation between Shp-2 (a cytoplasmic tyrosine phosphotase) and matrix metalloproteinase-1 (MMP-1) in the migration of mouse embryo fibroblast cell. Methods: Shp-2 -/-embryo fibroblast was separated from Shp-2 knockout mouse on E10. 5, and Shp-2 cDNA was transfected into Shp-2 -/- cell. The cell migration was observed with the wound healing, the MMP-1 expression and secretion was analyzed by immunoblotting and immunoprecipitation. The activation of MMP-1 in the supernatant was detected with type I collagenase activity assay after the stimulation of IL-1α compared with E10. 5 mouse embryo fibroblast cell(Shp-2 +/+). Results: There was obvious migration in Shp-2 +/+ cells and Shp-2 -/-R cells, but not in Shp-2 -/- cells. The shape of Shp-2 -/- cell was epithelial-like. The expression and secretion was increased in Shp-2 +/+ cells and Shp-2 -/-R cells, and it had not changed in Shp-2 -/- cell. The activation of MMP-1 was lower in Shp-2 -/- cells compared to the other cells. C Objective: To investigate the relation between Shp-2 (a cytoplasmic tyrosine phosphotase) and matrix metalloproteinase-1 (MMP-1) in the migration of mouse embryo fibroblast cell. Methods: Shp-2 - / - embryo fibroblast was separated from Shp- 2 knockout mouse on E10. 5, and Shp-2 cDNA was transfected into Shp-2 - / - cell. The cell migration was observed with the wound healing, the MMP-1 expression and secretion was analyzed by immunoblotting and immunoprecipitation. The activation of MMP-1 in the supernatant was detected with type I collagenase activity assay after the stimulation of IL-1α compared with E10. 5 mouse embryo fibroblast cell (Shp-2 + / +). Results: There was obvious migration in Shp-2 The shape of Shp-2 - / - cell was epithelial-like. The expression and secretion was increased in Shp-2 The activation of MMP-1 was lower in Shp-2 - / - cells compared to Sh cells and Shp-2 - / - R cells, and it had not changed in Shp-2 - / - cells. the other cells. C