肾穿刺活检组织常规特殊染色有PAS、PASM、Masson、PASM套染Masson法。传统的Masson染色法主要用于鉴别胶原纤维与肌纤维,而应用于肾穿刺活检标本的染色效果对比不鲜明、易褪色且染色效果不稳定。有文献报道,传统的Masson染色法经改良可得到较满意效果,但仍需经二次固定,时间长、操作繁琐~[1]。我科经过多年的反复摸索,对传统Masson染色法进行改良应用于肾穿刺活检组织,该改良方法染色时间短,无需二次固定,肾小球免 Kidney biopsy conventional special staining with PAS, PASM, Masson, PASM sets Masson method. The traditional Masson staining method is mainly used to identify collagen fibers and muscle fibers, and applied to the renal biopsy specimens staining contrast is not clear, easy to fade and the dyeing effect is not stable. It has been reported in the literature that the traditional Masson staining method can be more satisfactory after being modified, but it still needs to be fixed twice and takes a long time and the operation is cumbersome [1]. Our department after years of repeated exploration, the traditional Masson staining improved for renal biopsy, the improved method of dyeing time is short, without secondary fixation, glomerular