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目的了解广西肠道病毒的感染情况,为制定预防和控制肠道病毒感染策略提供依据。方法采用逆转录聚合酶链反应(RT-PCR)方法,对广西746例疑似肠道病毒感染者咽拭子、疱疹液和粪便标本共1062份进行肠道病毒、肠道病毒71型(EV71)和柯萨奇病毒A16型(CoxA16)核酸检测。结果共检出485份肠道病毒核酸阳性,阳性率为45.67%,其中EV71核酸阳性187份,阳性率为17.61%,40份为CoxA16核酸阳性标本,阳性率为3.77%。结论1062份标本中EV71核酸的阳性率高于CoxA16;粪便和疱疹液标本的阳性检出率高于咽拭子标本的阳性检出率;CoxA16和EV71是引起儿童手足口病的主要病原体,要加强对CoxA16和EV71的的鉴别诊断。
Objective To understand the prevalence of enterovirus infection in Guangxi and provide evidence for the prevention and control of enterovirus infection. Methods A total of 1062 samples of pharynx swab, herpes fluid and stool samples were collected from 746 suspected cases of enterovirus infection in Guangxi province by Enterovirus RT-PCR (RT-PCR) And Coxsackie virus type A16 (CoxA16) nucleic acids. Results A total of 485 positive samples of enterovirus nucleic acid were detected. The positive rate was 45.67%. Among them, 187 were positive for EV71, the positive rate was 17.61%, and 40 were positive for CoxA16. The positive rate was 3.77%. Conclusion The positive rate of EV71 nucleic acid in 1062 specimens was higher than that of CoxA16. The positive rate of stool and herpes fluid specimens was higher than that of throat swab specimens. CoxA16 and EV71 were the major pathogens causing hand-foot-mouth disease in children Enhance the differential diagnosis of CoxA16 and EV71.