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目的：研究血流脉动切应力对循环液中脂质过氧化物产生的影响。材料与方法：利用本室已建立的体外模拟脉动循环装置及硫代巴比妥酸测定法，进行 １９． ２９与 ５６． ２５ ｄｙｎｅｓ／ｃｍ~２两组切应力作用于培养人脐静脉内皮细胞０，１，２，４，６，１０，２４ ｈ后循环液中脂质过氧化物含量变化的实验。结果：在两组模拟脉动切应力的作用下，循环液中ＬＰＯ含量均于实验 １ｈ开始增加，４ ｈ达最高值，之后下降，于 ６ ｈ后下降趋于平稳，保持在一定的水平。但其中 ５６．２５ ｄｙｎｅｓ／ｃｍ~２切应力组循环液中 ＬＰＯ的含量明显高于 １９． ２９ ｄｙｎｅｓ／ｃｍ~２切应力组（Ｐ＜０．００１）。结论：血流脉动切应力作用于血管内皮细胞可以增加其循环液中脂质过氧化物的产生，随作用时间的延长，体内 ＬＰＯ的产生可维持在一定的水平，同时血流脉动切应力愈高促使脂质过氧化物产生的作用愈大。
Objective: To study the effect of pulsating shear stress on the production of lipid peroxides in circulating fluid. MATERIALS AND METHODS: The in vitro simulative pulsatile circulation device and thiobarbituric acid assay established in our laboratory were used. 29 and 56. The experiment of 25dynes / cm ~ 2 shear stress on the changes of lipid peroxides in the circulating fluid after culturing human umbilical vein endothelial cells at 0, 1, 2, 4, 6, 10 and 24 hours. Results: LPO content in circulating fluid increased from 1 h to 1 h after simulating pulsating shear stress, reaching the highest value at 4 h and then decreasing. After 6 h, the LPO content tended to be stable and remained at a certain level. However, the content of LPO in the circulation solution of 56.25 dynes / cm ~ 2 shear stress group was significantly higher than 19%. 29 dynes / cm ~ 2 shear stress group (P <0.001). Conclusion: The effect of pulsatile shear stress on vascular endothelial cells can increase the production of lipid peroxides in the circulating fluid. With the prolongation of the action time, the production of LPO in the body can be maintained at a certain level. At the same time, the pulsating shear stress High promote the role of lipid peroxides produce greater.