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目的 :证实先天性视网膜变性 (RCS)大鼠视网膜内核层存在神经原变性。方法 :使用出生后第 18,2 0 ,2 8,35 ,42 ,45 ,5 6 ,6 0 ,70和 10 0d的RCS大鼠视网膜和正常SD大鼠比较 ,利用视网膜内核层细胞标记物 :抗Calbindin ,PKC和Calretinin血清 ,经免疫组化方法分别标记水平细胞、杆双极细胞及无长突细胞 ,并统计这三种细胞的数目。结果 :和正常SD大鼠视网膜比较 ,RCS大鼠从 18～ 2 0d开始视网膜变性 ,除了外核层细胞死亡 ,内核层细胞也有不同程度的丢失 ,并表现为浓染或水肿。统计结果表明 ,内核层水平细胞数目从出生后 6 0d开始减少 ,杆双极细胞在出生后2 0d即数目已减少 ,但可保持至 10 0d才有进一步减少 ,无长突细胞虽然在出生后 2 0～ 35d数目明显减少 ,但可一直保持这个水平。结论 :RCS大鼠视网膜内核层细胞存在神经原变性 ,水平细胞、杆双极细胞、无长突细胞发生损失的时间不同 ,反映了中继神经原变性很可能是这些细胞变性的机理
PURPOSE: To demonstrate the presence of neurogenic degeneration in the nucleus of the retina of congenital retinal degeneration (RCS) rats. Methods: Compared with normal SD rats at the 18th, 20th, 28th, 35th, 42th, 45th, 56th, 60th, 70th, and 100th day after birth, Anti-Calbindin, PKC and Calretinin sera were used to detect the levels of horizontal cells, rod bipolar cells and amacrine cells by immunohistochemistry. The numbers of these three cells were counted. Results: Compared with the normal retina of rats, RCS rats developed retinal degeneration from 18 to 20 days. In addition to the death of outer nuclear layer cells, the inner nuclear cells were also lost to varying degrees and showed thick or edema. The statistical results showed that the number of horizontal cells in the inner nuclear layer decreased from 60 days after birth and the number of rod bipolar cells decreased after 20 days of birth but could be reduced to 100 days later. 2 0 ~ 35d number significantly reduced, but can maintain this level. CONCLUSIONS: There is neurogenic degeneration in the nuclei of retina of RCS rats, and the time of loss of horizontal cells, rod bipolar cells and amacrine cells is different, which suggests that the relaying of neurogenic degeneration may be the mechanism of degeneration of these cells