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从圭630/台湾粳的F_1花药培养后代群体中发现了水稻幼穗分化受阻突变体lhd(leafy head),其植株明显矮化,叶片细小且丛生,始终停留在营养生长阶段.遗传分析表明,lhd受一对隐性基因控制,该突变基因拟命名为lhd(t).显然,LHD(t)是控制花序分化的关键基因.以lhd杂合体与明恢77和京花8号杂交,建立了2个F_2群体.在与京花8号杂交的F_2群体中,部分lhd植株表现出“中间类型”,说明遗传背景会影响突变性状的表现.利用已公布的水稻RM系列SSR标记及自行设计的SSR标记,结合BSA和突变株(共498株)分析,将LHD(t)基因定位在第10染色体长臂端,其中标记SSR1,RM269,RM258,RM304和RM171位于一侧,与LHD(t)的图距分别为6.4,16.6,18.4,22.2和26.3 cM;SSR4和SSR5位于另一侧,与LHD(t)的图距分别为0.6和2.2 cM.该结果为进一步对LHD(t)的克隆和表达研究奠定了基础.
From the F1 generation of F1 progenies in Kyu-630 / Taiwan japonica, we found that leaf-tip mutant lhd (leafy head) was obviously dwarfed and the leaves were small and clustered and remained in the vegetative growth stage.Genetic analysis showed that, lhd is controlled by a pair of recessive genes, which are to be named lhd (t). It is clear that LHD (t) is a key gene controlling inflorescence differentiation and is characterized by hybridization of lhd hybrid with Minghui 77 and Jinghua 8 2 F_2 populations were observed.Among the F_2 populations crossed with Jinghua 8, some lhd plants showed “intermediate types”, indicating that the genetic background would affect the performance of the mutant traits.Based on the published rice RM series SSR markers and self-designed The markers of SSR1, RM269, RM258, RM304 and RM171 were located on one side of the long arm of chromosome 10 in combination with the analysis of 498 strains of BSA and LHD (t ) Were 6.4, 16.6, 18.4, 22.2 and 26.3 cM, respectively; SSR4 and SSR5 were located on the other side with 0.6 and 2.2 cM LHD (t) intervals, respectively. Cloning and expression studies laid the foundation.