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介绍一种利用普通光学显微镜直接分离玉米大斑病病菌单孢子的方法。该方法是通过敲打叶片先将孢子转移至水琼脂培养基上,然后利用自制的简易挑孢针在低倍镜下直接挑取大斑单孢子,从而达到分离纯化的目的。结果表明,此法虽然有一定的操作难度,但简便易行,污染少,高效快捷,为玉米大斑病病菌生理小种鉴定、DNA遗传多态性的进一步研究做了积极有益的基础工作。该方法值得推广到其他种类大型孢子真菌的单孢分离工作中。
This paper introduces a method of separating single spore of S. turcica by using ordinary light microscope. The method comprises the following steps of: firstly, spores are transferred onto water agar medium by means of beating leaves, and then the large single spore is directly taken under the low magnification by using a homemade simple spore picking needle so as to achieve the purpose of separation and purification. The results showed that although this method has a certain degree of operational difficulty, it is easy to operate, less polluting, more efficient and faster. It has made a positive and beneficial basic work for the identification of physiological races of S. turcica and DNA genetic polymorphism. This method is worth to be extended to the single spore separation of other large fungal spores.